A study on correlation between vaginal dysbacteriosis and intrauterine adhesion
SHEN Rongjiong
FAN Jieru
HUANG Huan
ZHAO Xingping
SHU Changfa
WANG Haiyan
Abstract：Objective To explore the correlation between vaginal dysbacteriosis and intrauterine adhesion(IUA).Methods Thirty patients with IUA who were treated in the Third Xiangya Hospital of Central South University from January 1,2022 to May 31,2022 were recruited as IUA group.Other 30 healthy women who underwent hysteroscopy at the same time to exclude IUA were selected as control group.A sample of secretions was collected at the midvagina using a sterile swab.The variable region of the 16S rRNA gene was amplified through polymerase chain reaction,and high-throughput sequencing was performed on the MiSeq Illumina sequencing platform.The sequencing data were processed through bioinformatics analysis method to analyze the bacterial diversity.Results The results of bacterial diversity analysis showed that the bacterial diversity in the IUA group was lower than that in the control group.The grade-abundance distribution curve suggested that the bacterial abundance in the midvagina was higher and the species were more evenly distributed in the control group.The Alpha diversity analysis results showed that the Chao index,ACE index,pedigree diversity index,Shannon's index and the number of observed species in the IUA group were significantly lower than those in the control group(P＜0.05),while the Simpson's index in the IUA group was significantly higher than that in control group(P＜0.05).The relative abundance of Firmicutes in the IUA group was significantly higher than that in the control group(P＜0.05),while the relative abundances of Actinomyces,Bacteroides,Proteus,Fusobacteria,Planctomycetes,verrucous microorganisms and G.oxydans in the IUA group were significantly lower than those in the control group(P＜0.05).The relative abundances of Lactobacillus prophages Lj928,Klebsiella pneumoniae and Roseburia inulinivorans in the IUA group were significantly higher than those in the control group(P＜0.05),while the relative abundances of Atopobium vaginae,Bifidobacteria,Pseudomonas,Prevotella,Aeromonas veronii,Shewanella xiamenensis,Bacteroides melaninogenicus,Porphyromonas and Phascolarctobacteriumin the IUA group were significantly lower than those in the control group(P＜0.05).Conclusion The richness and diversity of vaginal flora decrease significantly in IUA patients,which may be one of the mechanisms of IUA.Improving vaginal dysbacteriosis may contribute to the prevention and treatment of IUA.
Keywords：Intrauterine adhesion(IUA)Vaginal floraBiodiversityHigh-throughput nucleotide sequencing
Publication Date：2025-05-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 525-531 )