A study on effects of Garcinone C on lysosomal damage of nasopharyngeal carcinoma cells and their related mechanisms
DAI Yiping
YIN Fuqiang
WANG Chunlai
SUN Xuemei
ZHANG Jinyan
LIU Xia
Abstract：Objective To explore the effects of Garcinone C on lysosomal damage of nasopharyngeal carcinoma(NPC)cells and their related mechanisms.Methods NPC cells HONE1 and S18 were selected for experiment,and Garcinone C was used as intervention treatment.CCK-8 assay was used to detect cell proliferation viability,and the phosphate ester method was used to detect acid phosphatase activity.The expression levels of lysosomal-associated membrane protein 1(LAMP1),galectin-3(Gal-3)and cathepsin D(CTSD)were detected by using immunofluorescence assay,and their localizations were observed.The expression levels of CTSD,LAMP1,Gal-3,Ras-related protein Rab-7a(Rab7a)and vesicle-associated membrane protein 8(VAMP8)were detected by using Western blot.Results Garcinone C could inhibit the viability of NPC cells and the activity of acid phosphatase(P＜0.05),and promote an increase in the expression levels of LAMP1,Gal-3 and CTSD in the cytoplasm(P＜0.05).After intervention with Garcinone C,the expression levels of CTSD,LAMP1,Gal-3 and Rab7a proteins in NPC cells were significantly increased(P＜0.05),while the expression level of VAMP8 protein was significantly decreased(P＜0.05).These regulatory effects were dose-dependent.Conclusion Garcinone C may play a therapeutic role by damaging lysosomes of NPC cells.Garcinone C is expected to be a candidate lead compound in the development of chemotherapy drugs for NPC.
Keywords：Garcinone CNasopharyngeal carcinomaLysosomal damage
Publication Date：2025-04-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 396-401 )