Effects of female age at the time of oocyte cryopreservation on embryonic development and clinical pregnancy outcomes
HU Qingyun
JI Hui
ZHANG Junqiang
ZHAO Chun
LANG Peng
LING Xiufeng
Abstract：Objective To analyze the effects of female age at the time of oocyte cryopreservation on embryonic development and clinical pregnancy outcomes.Methods The clinical data of women who underwent oocyte vitrification freezing and thawing,and received intracytoplasmic sperm injection(ICSI)assisted pregnancy cycle in Women's Hospital of Nanjing Medical University from January 2013 to May 2024 were retrospectively analyzed.According to the women's age at the time of oocyte retrieval,they were divided into women＜35 years old group(67 cases)and women ≥35 years old group(21 cases).The general clinical data,embryonic development and clinical pregnancy outcomes were compared between the two groups.Results The level of body mass index(BMI)in the women ≥35 years old group was significantly higher than that in the women＜35 years old group(P＜0.05).There were no significant differences in basal follicle-stimulating hormone(FSH),basal estradiol(E2),basal progesterone(P),basal luteinizing hormone(LH),basal testosterone(T)and superovulation protocols between the two groups(P＞0.05).The level of LH on human chorionic gonadotropin(HCG)trigger day in the women≥35 years old group was significantly higher than that in the women＜35 years old group(P＜0.05),while there were no significant differences in the levels of E2 and P on HCG trigger day between the two groups(P＞0.05).There were no statistically significant differences between the two groups in oocyte survival rate,normal fertilization rate,oocyte degeneration rate after ICSI,2 pronuclei(2PN)cleavage rate,usable embryo rate,high-quality embryo rate and blastocyst formation rate(P＞0.05).After adjusting for confounders including BMI,endometrial thickness on HCG trigger day,embryo transfer stage,number of transferred embryos,and number of high-quality embryos transferred,the results of multivariate logistic regression analysis showed that compared with those＜35 years old,the women ≥35 years old had significantly lower probabilities of fresh embryo live birth and cumulative live birth(P＜0.05).Conclusion Increasing the female age at the time of oocyte cryopreservation may reduce the rate of live birth after embryo transfer.
Keywords：OocyteAge at the time of oocyte cryopreservationVitrification freezingClinical pregnancy rateLive birth rate
Publication Date：2025-03-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 279-284 )