Analysis on the allelic distribution and haplotype polymorphism of HLA-A,HLA-B,HLA-C,HLA-DRB1,HLA-DQB1 in the renal transplantation recipients of a single-center Han population in Shandong
SHAO Changfeng
KONG Yirong
WANG Liqin
WANG Rong
JU Qiang
CHEN Shumin
WANG Haiyan
Abstract：Objective To analyze characteristics of the allelic distribution and haplotype polymorphism of HLA-A,HLA-B,HLA-C,HLA-DRB1,HLA-DQB1 in the renal transplantation recipients of a single-center Han population in Shandong,and to obtain human leukocyte antigen(HLA)genetic parameters of the kidney transplant population.Methods A total of 1 470 renal transplantation recipients who underwent allele detection of HLA-A,HLA-B,HLA-C,HLA-DRB1 and HLA-DQB1 in the Affiliated Hospital of Qingdao University from June 2019 to December 2022 were selected.The method of polymerase chain reaction-sequence specific oligonucleotide probe(PCR-SSOP)was used for allele detection of HLA.The frequency of HLA alleles was calculated by using direct counting method,and Hardy-Weinberg equilibrium test was performed for the loci of each allele.The common haplotype frequencies were calculated by using Arlequin 3.5.2.2 software.Results A total of 216 alleles were detected in the 1 470 patients,among which 36,86,33,40 and 21 alleles were detected in HLA-A,HLA-B,HLA-C,HLA-DRB1 and HLA-DQB1,respectively.There were no significant differences between the observed values of HLA-A,HLA-B and HLA-C alleles and the expected values of HLA-A,HLA-B and HLA-C alleles(P＞0.05).There were significant differences between the observed values of HLA-DRB1 and HLA-DQB1 alleles and the expected values of HLA-DRB1 and HLA-DQB1 alleles(P＜0.05).There were a total of 452 haplotype pairs of HLA-A-HLA-B,219 haplotype pairs of HLA-B-HLA-C,666 haplotype pairs of HLA-A-HLA-B-HLA-C,551 haplotype pairs of HLA-B-HLA-DRB1,91 haplotype pairs of HLA-DRB1-HLA-DQB1,1 367 haplotype pairs of HLA-A-HLA-B-HLA-C-HLA-DRB1,and 1 434 haplotype pairs of HLA-A-HLA-B-HLA-C-HLA-DRB1-HLA-DQB1.HLA-A*30:01-HLA-B*13:02-HLA-C*06:02-HLA-DRB1*07:01-HLA-DQB1*02:02 haplotype frequency was the highest.Conclusion HLA-A*30:01-HLA-B*13:02-HLA-C*06:02-HLA-DRB1*07:01-HLA-DQB1*02:02 haplotype frequency is the highest in the renal transplantation recipients of the Han population in Shandong.This study provides a reference for donor allele search and recipient population genetics research.
Keywords：Human leukocyte antigen(HLA)Renal transplantationAlleleHaplotype
Publication Date：2025-01-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 71-76 )