Analysis on the correlation of serum SCUBE1 and αvβ3 expressions with sensitivity to clopidogrel after PCI surgery in patients with coronary heart disease
CHEN Juan
WANG Xiaoli
WU Shuhua
YUAN Yongcheng
QIN Jiangjun
Abstract：Objective To analyze the expressions of signal peptide-CUB-EGF-like domain-containing protein 1(SCUBE1)and integrin αvβ3 in the serum of patients with coronary heart disease(CHD),and to analyze the correlation between these expressions and sensitivity to clopidogrel after percutaneous coronary intervention(PCI)surgery.Methods A total of 164 CHD patients admitted to Sanya Central Hospital from March 2022 to September 2023 were recruited as CHD group,and 150 healthy individuals undergoing physical examination during the same period were selected as control group.According to inhibition rate of platelet aggregation(IPA),the CHD patients were divided into low-sensitive group(41 cases,IPA＜20％)and high-sensitive group(123 cases,IPA ≥20％).Enzyme-linked immunosorbent assay(ELISA)was used to detect serum SCUBE1 and αvβ3 levels in the control group on the day of physical examination and in the CHD group before PCI surgery,immediately after PCI surgery,and 48 hours after PCI surgery.Multivariate logistic regression was used to analyze the factors affecting the decrease of clopidogrel sensitivity in the CHD patients after PCI surgery.Receiver operating characteristic(ROC)curve was used to analyze the predictive efficiency of preoperative serum SCUBE1 andαvβ3 levels for the decrease of clopidogrel sensitivity in the CHD patients after PCI surgery.Results The serum SCUBE1 and αvβ3 levels before PCI surgery,immediately after PCI surgery,and 48 hours after PCI surgery in the CHD group were significantly higher than those in the control group(P＜0.05),and the postoperative serum SCUBE1 and αvβ3 levels showed an upward trend,and the differences in intra-group comparisons between different time points were statistically significant(P＜0.05).The serum SCUBE1 and otvβ3 levels before PCI surgery,immediately after PCI surgery,and 48 hours after PCI surgery in the low-sensitive group were higher than those in the high-sensitive group,and the differences were statistically significant between the two groups(P＜0.05).The results of multivariate logistic regression analysis showed that preoperative higher levels of platelet(PLT)[OR(95％CI)=2.693(1.182-6.134)],mean platelet volume(MPV)[OR(95％CI)=2.285(1.328-3.933)],SCUBE1[OR(95％CI)=2.571(1.352-4.890)],and αvβ3[OR(95％CI)=3.254(1.113-7.340)]were independent risk factors for promoting the decrease in sensitivity to clopidogrel after PCI surgery(P＜0.05).The results of ROC curve analysis showed that both serum SCUBE1 and αvβ3 levels before surgery could effectively predict the decrease of clopicogrel sensitivity in the CHD patients after PCI surgery,and the combined predictive efficiency of the two indicators was higher[AUC(95％CI)=0.899(0.842-0.940)],with sensitivity of 72.61％and specificity of 94.31％.Conclusion The serum SCUBE1 and αvβ3 levels of CHD patients increase,and preoperative serum SCUBE1 and αvβ3 levels can effectively predict the decrease of clopidogrel sensitivity after PCI surgery,which is worth the attention of clinicians.
Keywords：Coronary heart disease(CHD)Percutaneous coronary intervention(PCI)ClopidogrelSignal peptide-CUB-EGF-like domain-containing protein 1(SCUBE1)Integrin αvβ3
Publication Date：2025-01-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 41-46 )