An exploration on the expression of TSC22D1 in oropharyngeal squamous cell carcinoma and its correlation with HPV infection
LIU Nengming
CHEN Pengning
CHEN Xinyu
CHEN Guanxi
LUO Lin
ZHOU Bosen
ZHOU Mengyu
RUAN Ping
YU Dahai
Abstract：Objective To analyze the expression of TSC22D1 in oropharyngeal squamous cell carcinoma(OPSCC) and to explore its correlation with human papilloma virus(HPV) infection(p16+).Methods The clinical data and tissue specimens of 55 patients with OPSCC were retrospectively analyzed,including 27 cases of tonsil cancer,25 cases of tongue root cancer and 3 cases of soft palate cancer.The tissue specimens of 12 cases of tongue squamous cell carcinoma(TSCC) were collected for analysis,and 11 cases of specimens of tonsillitis and 2 cases of specimens of paracarcinomatous tongue epithelium were used as controls.Immunohistochemical staining was used to detect the expressions of TSC22D1 and p16.The relationship between the expression of TSC22D1 and the clinicopathological indicators of OPSCC patients was analyzed.Results TSC22D1 was continuously expressed in the columnar epithelial zone at the opening of Von Ebner's glandular ducts as well as in the basal layer of the transformation zone in the paracarcinoma tongue,and was only sporadically expressed in basal cells in the epithelial tissues of the paracarcinoma tongue,and was densely expressed in some TSCCs and carcinomas of the tongue root.There was no significant difference in the expression of TSC22D1 between TSCC tissues and the tissues of tongue root carcinomas(P＞0.05).TSC22D1 was continuously expressed in the reticular epithelium of tonsils and was positively expressed in some tonsillar carcinomas.The positive expression rate of TSC22D1 in non-cancerous tonsil tissues( tonsillitis tissues) was higher than that in cancerous tonsil tissues,and the difference was statistically significant(P＜0.05).The low differentiation rate of TSC22D1-positive OPSCC was significantly higher than that of TSC22D1-negative OPSCC(P＜0.05),and the TSC22D1 expression was not significantly associated with the patients' gender,age,number of cigarettes smoked,ethnicity,site of disease,N stage,T stage,clinical stage and HPV infection(p16+)(P＞0.05).Conclusion TSC22D1 may be a susceptible regional marker of OPSCC,and its expression reduces in carcinogenesis of oropharynx.TSC22D1 may not be significantly associated with the expression of p16.
Keywords：TSC22D1Oropharyngeal squamous cell carcinoma(OPSCC)Human papilloma virus(HPV)
Publication Date：2024-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1277-1282 )