Analysis on the expression of differentially expressed miRNA in osteosarcoma patients and its correlation with survival prognosis

LIU Qi; QIAN Jun; CAO Juan; YANG Qian

doi:10.3969/j.issn.1674-3806.2024.11.11

Abstract：Objective To analyze the expression of differentially expressed miRNA in osteosarcoma(OS) patients and its correlation with survival prognosis.Methods The bioinformatics analysis method was used to identify differentially expressed miRNA in GSE69470 and GSE70367 datasets.Sixty-three OS patients who visited their doctors in Zhang Ye People's Hospital Affiliated to Hexi University from August 2018 to January 2019 were recruited.Real-time fluorescence quantitative polymerase chain reaction( RT-qPCR) was used to detect the expression levels of differentially expressed miRNA in OS tissues and their paracancer tissues,and the expression level of differentially expressed miRNA in the OS tissues was compared with that in the paracancer tissues.The patients were followed up by telephone or outpatient reexami-nation methods and their survival situation was recorded.The patients were followed up for 5 years until January 2024.The Kaplan-Meier method was used to analyze the correlation between the differentially expressed miRNA expression in OS tissues and the survival prognosis of the OS patients.Results Eight differentially expressed miRNA were obtained by bioinformatics analysis,including microRNA(miR)-543,miR-495-3p,miR-433-3p,miR-381-3p,miR-301a-3p,miR-199b-5p,miR-125b-5p and miR-218.The results of RT-qPCR showed that the relative expression levels of miR-543,miR-495-3p and miR-125b-5p in the OS tissues were lower than those in the paracancer tissues,while the relative expression levels of miR-433-3p,miR-381-3p,miR-301a-3p and miR-199b-5p in the OS tissues were higher than those in the paracancer tissues,and the differences were statistically significant(P＜0.05).There was no statistically significant difference in the expression level of miR-218 between the OS tissues and the paracancer tissues(P＞0.05).The patients were grouped according to the median relative expression of each miRNA:31 cases in the high expression group(＞median value) and 32 cases in the low expression group(≤median value).The analysis results of Kaplan-Meier method showed that the survival prognosis in the miR-543 high expression group,the miR-495-3p high expression group and the miR-125b-5p high expression group were better than that in the miR-543 low expression group,the miR-495-3p low expression group and the miR-125b-5p low expression group,respectively,and that the survival prognosis in the miR-433-3p low expression group,the miR-381-3p low expression group,the miR-301a-3p low expression group and the miR-199b-5p low expression group was better than that in the miR-433-3p high expression group,the miR-381-3p high expression group,the miR-301a-3p high expression group and the miR-199b-5p high expression group,respectively,and the differences were statistically significant(P＜0.05).Conclusion The relative expression levels of miR-543,miR-495-3p and miR-125b-5p in the OS tissues are lower than those in the paracancer tissues,while the relative expression levels of miR-433-3p,miR-381-3p,miR-301a-3p and miR-199b-5p in the OS tissues are higher than those in the paracancer tissues.The above 7 kinds of differentially expressed miRNA are associated with the survival prognosis of the OS patients.