A study on the anti-photoaging effect of dental pulp stem cells-conditioned medium combined with LDH nanomaterials on HaCaT cells
QIU Liuliu
XIE Shenxia
YANG Xiaomei
LU Xiaoling
Abstract：Objective To explore the effect of dental pulp stem cells-conditioned medium(DPSCs-CM)combined with layered double hydroxide(LDH)nanomaterials on photoaging HaCaT cells.Methods DPSCs-CM was prepared and LDH nanomaterials were synthesized.The control group[no pretreatment,no exposure to ultraviolet B(UVB)],model group(no pretreatment,exposure to UVB),DPSCs-CM group(undergoing UVB irradiation after 24-hour pretreatment with DMEM complete medium containing DPSCs-CM),LDH group(undergoing UVB irradiation after 24-hour pretreatment with DMEM complete medium containing LDH)and DPSCs-CM+LDH group(undergoing UVB irradiation after 24-hour pretreatment with DMEM complete medium containing DPSCs-CM and LDH mixed in equal proportion)were set up.The content of malondialdehyde(MDA),the activity of superoxide dismutase(SOD)and the level of reactive oxygen species(ROS)of HaCaT cells were detected in each group.The migration ability of HaCaT cells in each group was detected by using scratch wound healing assay.The expression levels of p53 protein and p16 protein of HaCaT cells in each group were detected by using Western blot method.Results The average particle size of the LDH nanomaterials was 65.52 nm,with a polydispersity index(PDI)of 0.109 and a Zeta potential of 38.1 mV,indicating that LDH nano-materials had low cytotoxicity,uniform particle size distribution,and good dispersion and stability.Compared with the model group,the DPSCs-CM group,LDH group and DPSCs-CM+LDH group had the low level of ROS and low content of MDA,high activity of SOD,the enhanced migration ability of HaCaT cells and reduced expression levels of intra-cellular p53 protein and p16 protein,and the differences were statistically significant(P＜0.05).The intervention effect of the DPSCs-CM+LDH group was the best.Conclusion DPSCs-CM combined with LDH nanomaterials can effectively prevent photoaging of HaCaT cells,and its effect is better than that of single DPSCs-CM intervention or LDH nanoma-terials intervention,which provides a reference for the research and development of skin photoaging treatment methods.
Keywords：Dental pulp stem cells-conditioned medium(DPSCs-CM)Layered double hydroxide(LDH)NanomaterialUltraviolet B(UVB)HaCaT cellPhotoaging
Publication Date：2024-09-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 992-997 )