A study on the correlation of serum levels of lncRNA-XIST and lncRNA-MALAT1 with insulin resistance and pregnancy outcome after ovulation induction therapy in infertile patients with polycystic ovary syndrome

LEI Jinmei; FENG Yuhuan; ZHANG Ou; GAO Shang; CHEN Wei

doi:10.3969/j.issn.1674-3806.2024.08.12

Abstract：Objective To explore the correlation of long noncoding RNA(lncRNA)X-inactive specific tran-script(XIST)(lncRNA-XIST)and lncRNA-MALAT1 with insulin resistance and pregnancy outcome after ovulation induction therapy in infertile patients with polycystic ovary syndrome(PCOS).Methods A total of 125 infertile patients with PCOS who underwent ovulation induction therapy in Tangshan Maternal and Child Health Hospital from February 2020 to January 2022 were recruited as PCOS infertility group,and other 125 healthy women who underwent physical examination during the same period were selected as control group.The serum levels of lncRNA-XIST and lncRNA-MALAT1,and homeostasis model assessment-insulin resistance(HOMA-IR)index were compared between the two groups.Pearson correlation analysis was used to explore the correlation of serum levels of lncRNA-XIST and lncRNA-MALAT1 with HOMA-IR.Multivariate logistic regression analysis was used to explore the factors affecting the pregnancy outcome after ovulation induction therapy in infertile patients with PCOS.Results The serum levels of lncRNA-XIST and HOMA-IR in the PCOS infertility group were higher than those in the the control group,and the lncRNA-MALAT1 level in the PCOS infertility group was lower,and the differences were statistically significant(P＜0.05).HOMA-IR was positively correlated with serum IncRNA-XIST level(r=0.689,P＜0.001),and negatively correlated with serum lncRNA-MALAT1 level(r=-0.771,P＜0.001).The cumulative clinical pregnancy rate of the PCOS infertility patients after ovulation induction therapy was 43.20％(54/125).The results of multivariate logistic regression analysis showed that higher luteinizing hormone(LH)/follicle stimulating hormonec(FSH)ratio[OR(95％CI)=1.121(1.005-1.278)]and serum level of lncRNA-XIST[OR(95％CI)=1.252(1.014-1.449)]were independent risk factors for promoting non-pregnancy in the PCOS infertility patients after treatment(P＜0.05),and higher serum levels of anti-Müllerian hormone(AMH)[ORR(95％CI)=0.518(0.348-0.771)]and lncRNA-MALAT1[OR(95％CI)=0.394(0.238-0.652)]were pro-tective factors for promoting pregnancy in the PCOS infertility patients after treatment(P＜0.05).Conclusion Serum lncRNA-XIST expression is high and lncRNA-MALAT1 expression is low in PCOS infertility patients,and the levels of the two indicators are closely related to insulin resistance and pregnancy outcome after ovulation induction therapy.