Expressions of Pin1 and paxillin proteins in lung cancer tissues and their clinical significance
LI Na
LIU Cunyu
HE Zhiwei
Abstract：Objective To explore the differences in the expressions of Pin1 and paxillin proteins in lung cancer tissues and paracancerous normal tissues,and to analyze their correlations with clinicopathological features.Methods The medical record data and pathological tissues of 208 patients with primary lung cancer who were admitted the Second Affiliated Hospital of Guangzhou Medical University from June 2018 to September 2022 were collected.Immunohisto-chemical staining(SP method)was used to detect the expressions of Pin1 and paxillin in the tissues,and their corre-lations with clinicopathological features was analyzed.Western blot was used to detect the expression levels of Pin1 and paxillin proteins in multiple lung cancer cell line cells.Kaplan-Meier survival curve was used to evaluate the cor-relations of Pin1 and paxillin expressions with the patients'prognosis.Results The levels of Pin1 and paxillin expres-sions in lung cancer tissues were higher than those in paracancerous normal tissues,and the differences were statisti-cally significant(P＜0.05).The expression of Pin1 protein was correlated with T stage,lymph node metastasis and clinical stage(P＜0.05),and the expression of paxillin protein was correlated with histological type and lymph node metastasis(P＜0.05).The results of Spearman rank correlation analysis showed a positive correlation between Pin1 protein expression and paxillin protein expression in lung cancer tissues(rs=0.455,P＜0.001).The results of Western blot showed that the expressions of Pin1 and paxillin varied in different lung cancer cell lines.The survival prognosis of the patients in the paxillin low expression group was significantly better than that of the patients in paxillin high expression group(P＜0.05),and the level of Pin1 expression was not significantly correlated with the survival prognosis of the lung cancer patients(P＞0.05).Conclusion The expressions of Pin1 and paxillin proteins are increased in lung cancer tissues,which are correlated with clinicopathological features.There is a positive correlation between Pin1 expression and paxillin expression in lung cancer tissues,and the level of paxillin expression can be used as one of the important indicators in judging the prognosis of lung cancer patients.
Keywords：Lung cancerPin1PaxillinPrognosisCorrelation
Publication Date：2024-07-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 721-727 )