A study on the expression of miR-4516 in nasopharyngeal carcinoma and its clinicopathological significance

HUANG Yongta; LI Yanyan; ZHANG Lixing; LIU Xia; ZHANG Shaojie; MO Xianglan; YIN Wu

doi:10.3969/j.issn.1674-3806.2024.05.16

Abstract：Objective To explore the expression of miR-4516 in nasopharyngeal carcinoma and its clinico-pathological significance.Methods The clinicopathological data of 60 patients with nasopharyngeal Epstein-Barr virus-related non-keratosis carcinoma(nasopharyngeal carcinoma group)who were admitted to the People's Hospital of Guangxi Zhuang Autonomous Region from February 2010 to October 2016 were retrospectively analyzed,and 15 patients with chronic nasopharyngeal mucosa inflammation during the same period were selected as control group.Real-time quantitative poly-merase chain reaction(RT-PCR)was used to detect the expression level of micro ribonucleic acid-4516(miR-4516)in the mucosal lesion tissues of the two groups and the results were compared between the two groups.The correlation between miR-4516 expression level and the clinicopathological features and survival prognosis of the nasopharyngeal carcinoma patients was analyzed.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic efficacy of miR-4516 in diagnosis of nasopharyngeal carcinoma metastasis.Results The expression level of miR-4516 in the nasopharyngeal carcinoma group was higher than that in the control group,and the difference was statistically significant(Z=4.477,P＜0.001).The expression level of miR-4516 in the mucosal lesion tissues of the nasopharyngeal carcinoma patients with metastasis was higher than that in the patients without metastasis,and the difference was statistically sig-nificant(P＜0.05).The expression level of miR-4516 was not significantly associated with the patients'gender,age,tumor site,T stage,and clinical stage(P＞0.05).The results of ROC curve analysis showed that miR-4516 could be used in diagnosing the metastasis of nasopharyngeal carcinoma[AUC(95％CI)=0.696(0.541-0.851),P=0.037],and the optimal cut-off value was 6.28,and the corresponding sensitivity and specificity were 0.63 and 0.75,respectively.The survival prognosis of the low miR-4516 expression group was significantly better than that of the high miR-4516 expression group(log-rank test:x=4.230,P=0.040).Conclusion The expression level of miR-4516 is up-regulated in nasopharyngeal carcinoma tissues,which can be used as a molecular marker to predict metastasis and clinical prog-nosis evaluation of nasopharyngeal carcinoma.