The value of circulating tumor cells combined with serum integrin β1 in diagnosis of intraductal malignant lesions of mammary glands in patients with pathological nipple discharge
ZHANG Teng
ZHANG Chao
LI Zhi
YU Man
REN Xinling
TIAN Ziru
SHI Lihui
Abstract：Objective To explore the diagnostic value of circulating tumor cells(CTCs)combined with inte-grin β1(ITG β1)in pathological nipple discharge(PND)patients with intraductal malignant lesions of mammary glands.Methods The clinical data of 212 patients with PND who were admitted to Beijing Tongzhou Maternal and Child Health Hospital and Beijing Chaoyang Hospital,Capital Medical University from January 2022 to September 2023 were retrospectively analyzed.The research subjects were divided into benign lesion group(187 cases)and malignant lesion group(25 cases)according to the results of pathological examination of mammary ducts.The number of CTCs in the PND patients was detected by using subtraction enrichment and immunostaining-fluorescence in situ hybridization(SE-iFISH),and the serum ITG β1 level was detected by using enzyme-linked immunosorbent assay(ELISA).Multivariate logistic regression was used to analyze the influencing factors of intraductal malignant lesions of mammary glands in the PND patients.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic efficacy of the number of CTCs and serum ITG β1 level on intraductal malignant lesions of mammary glands in the PND patients.Results There were sig-nificant differences in age,body mass index(BMI),menstrual status,type of discharge and breast mass between the two groups(P＜0.05).The number of CTCs in the malignant lesion group was larger than that in the benign lesion group,and the serum ITG β1 level in the malignant lesion group was higher than that in the benign lesion group,and the differ-ences were statistically significant(P＜0.05).The results of multivariate logistic regression analysis showed that age more than 50 years,BMI more than 24 kg/m2,blood discharge,more CTCs and higher serum ITG β1 level were inde-pendent risk factors for developing intraductal malignant lesions of mammary glands in the PND patients(P＜0.05).Both the number of CTCs and the serum ITG β1 level had the application value in the diagnosis of intraductal malignant lesions of mammary glands in the PND patients(P＜0.05),and the combined detection of the two indicators had better diagnostic efficacy[AUC(95％CI)=0.961(0.925-0.983),P＜0.001],and the sensitivity and specificity were 96.00％and 84.49％,respectively.Conclusion The number of CTCs and serum ITG β1 level are significantly elevated in PND patients with intraductal malignant lesions of mammary glands.The number of CTCs combined with serum ITG β1 level has better diagnostic value for intraductal malignant lesions of mammary glands in the PND patients.
Keywords：Pathological nipple discharge(PND)Intraductal malignant lesions of mammary glandsCirculating tumor cells(CTCs)Integrin β1(ITG β1)Diagnostic value
Publication Date：2024-05-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 543-547 )