Expressions of YTHDF1 and EIF3C in epithelial ovarian cancer and their clinical significance
FENG Shixiang
WANG Guanghui
CHEN Ting
ZHANG Jia
ZHAO Na
Abstract：Objective To analyse the expressions of YTH domain N6-methyladenosine RNA binding protein F1(YTHDF1)and eukaryotic initiation factor 3C(EIF3C)in epithelial ovarian cancer(EOC)and their clinical significance.Methods The clinical data of 130 patients who were itially diagnosed with EOC and treated in the Affiliated Hospital of Xuzhou Medical University from January 2016 to January 2020 were retrospectively analyzed,and the normal ovarian tissues of 70 patients who underwent surgical treatment for benign ovarian cysts during the same period were selected as controls.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the levels of YTHDF1 mRNA and EIF3C mRNA expressions in the tissues.Immunohistochemical staining was used to detect the expressions of YTHDF1 protein and EIF3C protein in the tissues.The correlation between the levels of YTHDF1 mRNA and EIF3C mRNA expres-sions was explore by using Pearson correlation analysis.The correlation between the expressions of YTHDF1 protein and EIF3C protein and the clinicopathological characteristics of EOC patients were analyzed.Kaplan-Meier method was used to plot survival curve.Cox regression was used to analyze the influencing factors of prognosis in the EOC patients.Results The levels of YTHDF1 mRNA and EIF3C mRNA expressions in the EOC tissues were higher than those in the normal ovarian tissues,and the differences were statistically significant(P＜0.05).The positive rates of YTHDF1 protein and EIF3C protein expressions in the EOC tissues were higher than those in the normal ovarian tissues,and the differ-ences were statistically significant(P＜0.05).The results of Pearson correlation analysis showed that the expression of YTHDF1 mRNA was positively correlated with that of EIF3C mRNA in the EOC tissues(r=0.802,P＜0.001).The expressions of YTHDF1 protein and EIF3C protein in the EOC tissues were significantly correlated with International Federation of Gynecology and Obstetrics(FIGO)stage,pathological grade and lymph node metastasis(P＜0.05).The survival prognosis of the negative YTHDF1 patients was better than that of the positive YTHDF1 patients(log rank test:x2=6.120,P=0.013).The survival prognosis of the negative EIF3C patients was better than that of the positive EIF3C patients(log rank test:=10.610,P=0.001).The results of Cox regression analysis showed that FIGO stage Ⅲ,patho-logical grade Ⅲ,lymph node metastasis,higher levels of carbohydrate antigen 125(CA125),and positive expressions of YTHDF1 protein and EIF3C protein were independent risk factors for poor prognosis of the EOC patients.Conclusion The expressions of YTHDF1 and EIF3C are elevated in the EOC tissues,which is associated with adverse clinicopath-ological characteristics.YTHDF1 and EIF3C are potential biomarkers for evaluating the prognosis of EOC.
Keywords：Epithelial ovarian cancer(EOC)YTH domain N6-methyladenosine RNA binding protein F1(YTHDF1)Eukaryotic initiation factor 3C(EIF3C)Prognosis
Publication Date：2024-05-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 531-537 )