A study on the expressions of FTO and GSDMD in gastric cancer tissues and their mechanisms of action
HUANG Shanpei
WEI Erdan
ZHU Liye
NING Qiting
CHEN Xingmei
CHEN Ni
WU Jiangni
QIU Xinze
HUANG Jie'an
LIU Shiquan
Abstract：Objective To explore the expressions of fat mass and obesity-associated protein(FTO)and gas-dermin D(GSDMD)in gastric cancer(GC)tissues and their mechanisms of action.Methods The differential expres-sions of FTO and GSDMD in GC tissues were analyzed by using the Tumor Immune Estimation Resource(TIMER)2.0 database.Forty-five GC tissues and the corresponding paracancer tissues(3-5 cm away from the tumor margin)which were surgically excised from the patients in the Second Affiliated Hospital of Guangxi Medical University from January 2022 to March 2023 were collected,and the patients'clinicopathological data were collected.Immunohistochemical staining method was used to detect the expressions of FTO and GSDMD in the tissues,and their correlations with the patients'clinicopathological features were analyzed.AGS cells with low expression of FTO were constructed by transfecting low expression of FTO lentivirus(FTO low expression group),and the cells transfected with empty vector lentivirus were set up as the control group.Real-time fluorescence quantitative polymerase chain reaction(RT-PCR)was used to detect the levels of FTO mRNA and GSDMD mRNA expressions in the cells in the two groups.Cell scratch wound healing assay and cell migration test were used to analyze the cell migration ability in the two groups.Results The analysis results based on TIMER 2.0 database and the immunohistochemical staining results showed that FTO and GSDMD were highly expressed in GC tissues(P＜0.05).The high expressions of FTO and GSDMD were closely related to the depth of tumor invasion and lymph node metastasis(P＜0.05).The results of cell experiment showed that the level of GSDMD mRNA expression in the FTO low expression group was significantly lower than that in the control group(P＜0.05),and the scratch healing rate was lower and the number of cell migration was less in the FTO low expression group,and the differences were statistically significant(P＜0.05).Conclusion FTO may promote the metastasis of GC cells by reg-ulating the expression of GSDMD.
Keywords：Gastric cancer(GC)Fat mass and obesity-associated protein(FTO)Gasdermin D(GSDMD)Cell migration
Publication Date：2024-04-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 400-406 )