Exploration on the application value of MHC-Ⅱ combined with MHC-Ⅰ immunohistochemical staining in diagnosis of idiopathic inflammatory myopathy
DUO Jianying
DI Li
LU Yan
WANG Min
HUANG Yue
ZHU Wenjia
WEN Xinmei
XU Min
CHEN Hai
DA Yuwei
Abstract：Objective To explore the application value of major histocompatibility complex(MHC)-Ⅱcom-bined with MHC-Ⅰ immunohistochemical staining in diagnosis of idiopathic inflammatory myopathy(IIM).Methods A total of 29 specimens were collected from the patients who received inmuscle biopsy in the Department of Neurology of Xuanwu Hospital Capital Medical University during March 2010 and April 2018,and the clinical data of the patients were collected through the hospital electronic medical record system,including 4 types of IIM[dermatomyositis(DM)(5 cases),polymyositis(PM)(5 cases),sporadic inclusion body myositis(IBM)(4 cases)and necrotizing antoimmune myopathy(NAM)(5 cases)]and 2 types of non-inflammatory myopathy(NIM)[muscular dystrophy(MD)(5 cases),dysferlinopathy myopathy(5 cases)].Hematoxylin-eosin(HE)staining and MHC-Ⅰ and MHC-Ⅱ immunohistochemical staining were performed on the specimens.Results The results of immunohistochemical staining showed that the positive rate of MHC-Ⅰ in the muscle specimens of PM,DM and IBM patients was 100.0%,and the positive rate of MHC-Ⅰin the muscle specimens of NAM and MD patients was 80.0%,and the positive rate of MHC-Ⅰin the muscle specimens of dysferlinopathy myopathy patients was 20.0%.The positive rate of MHC-Ⅱ in the muscle specimens of PM and IBM patients was 40.0%and 50.0%,respectively.The immunohistochemical staining of MHC-Ⅱ in the muscle specimens of the patients with other types of diseases was negative.Conclusion MHC-Ⅱ staining has higher specificity than MHC-Ⅰ staining in distin-guishing IIM from NIM,and MHC-Ⅱcombined with MHC-Ⅰimmunohistochemical staining has better clinical appli-cation value in the diagnosis of different types of myopathy.
Keywords：Idiopathic inflammatory myopathy(IIM)Major histocompatibility complex-Ⅱ(MHC-Ⅱ)Major histocompatibility complex-Ⅰ(MHC-Ⅰ)Immunohistochemical staining
Publication Date：2024-03-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 277-282 )