An exploration on the effect of miR-3189-3p on prognosis of patients with colorectal cancer and its mechanism of action
CAO Yusheng
LIANG Haoyuan
LI Lintao
JIANG Kaihai
HUANG Tianfu
LI Guangsheng
HUANG Xusen
Abstract：Objective To explore the effect of micro ribonucleic acid-3189-3 p(miR-3189-3 p)on prognosis of patients with colorectal cancer(CRC)and its mechanism of action.Methods Based on the data of CancerMIRNome database and ENCORI database,the differences in the expressions of miR-3189-3p in CRC tissues and normal tissues were analyzed,and the effect of the level of miR-3189-3p expression on the survival prognosis of the patients was explored.Hsa-miR-3189-3p mimics and miR-3189-3p NC were transfected into LOVO cells,respectively.The effects of miR-3189-3p on the expression levels of the key proteins(vimentin and N-cadherin)of epithelial-mesenchymal transition(EMT)in the LOVO cells were explored by using Western blot assay.The effects of miR-3189-3p on the migration and invasion ability of the LOVO cells were explored by using scratch assay and Transwell assay.Results The level of miR-3189-3p expression in the CRC tissues was significantly higher than that in the normal tissues(P＜0.05).The survival prognosis of the CRC patients with high expression of miR-3189-3p was better than that of the CRC patients with low expression of miR-3189-3p(P＜0.05).After overexpression of miR-3189-3 p,the migration and invasion ability of the LOVO cells were significantly weakened(P＜0.05),and the expression levels of vimentin and N-cadherin proteins were signifi-cantly decreased(P＜0.05).Conclusion MiR-3189-3p may affect the survival prognosis of the patients by regulating the migration,invasion and EMT of CRC cells.
Keywords：Micro ribonucleic acid-3189-3p(miR-3189-3p)Colorectal cancer(CRC)Epithelial-mesenchymal transition(EMT)MigrationInvasion
Publication Date：2024-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 206-211 )