Construction of Wharton's jelly-modified polycaprolactone electrospinning artificial blood vessel scaffolds and analysis of their performances
HE Fangling
JIANG Tingbo
Abstract：Objective To construct Wharton's jelly(WJ)-modified polycaprolactone(PCL)electrospinning arti-ficial blood vessel scaffolds and to analyze their performances.Methods The WJ tissues were decellularized by using trypsin method,and histological staining and biochemical component quantification analysis were employed to analyze the efficiency of WJ tissue decellularization and the differences in the biochemical components of the WJ tissues before and after decellularization.Enzyme-linked immunosorbent assay(ELISA)was used to detect the differences in the levels of bioactive factors of the WJ tissues before and after decellularization.The processed WJ was mixed with PCL to prepare spinning solution,and the electrospinning technique was utilized to fabricate tubular WJ/PCL electrospinning artificial blood vessel scaffolds,and their nano-fiber structures,fiber diameter distributions,biomechanical properties and blood compatibilities were analyzed.Results Trypsin-based decellularization effectively removed cellular nuclear components from WJ matrix,preserving most of the biochemical components of the WJ matrix[including glycosaminoglycan(GAG),proteins,fibers,hyaluronic acid,and hydroxyproline]and the bioactive factors of the WJ matrix[including vascular endo-thelial growth factor(VEGF),platelet-derived growth factor(PDGF),transforming growth factor-beta 1(TGF-β1),and fibroblast growth factor-2(FGF-2)].The electrospinning WJ/PCL artificial blood vessel scaffolds prepared by the elec-trospinning technique exhibited a typical nano-fiber structure,and their characteristic peaks of both WJ and PCL were ver-ified by Fourier-transform infrared spectroscopy(FTIR).WJ/PCL electrospinning artificial blood vessel scaffolds demonstrated good biomechanical properties and blood compatibilities,effectively inhibiting platelet adhesion.Conclusion WJ is an ideal natural material that can be used in combination with synthetic materials like PCL to prepare electrospinning artificial blood vessel scaffolds with desirable biological properties.
Keywords：Wharton's jelly(WJ)ElectrospinningComposite bio-scaffoldArtificial blood vessel
Publication Date：2024-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 179-185 )