Analysis of the effects of three detection methods on diagnosis of mycobacterium infection
LEI Zhen
YANG Xiao-bing
TANG Liu-sheng
Abstract：Objective To analyze the diagnostic effects of polymerase chain reaction(PCR)fluorescence probe method,BD960 liquid culture method and direct smear fluorescence staining method on mycobacterium infection.Methods From January 2021 to April 2021,104 patients with suspected mycobacterium who visited the doctors infection in Chest Hospital of Guangxi Zhuang Autonomous Region were selected as the research subjects.The BD960 liquid culture method was used as the reference to evaluate the detection efficiency of PCR-fluorescence probe method and direct smear fluo-rescence staining method in detection of mycobacteria.Results In the specimens of the 104 patients with suspected mycobacterial disease,there were significant differences in the detection results between the BD960 liquid culture method and PCR-fluorescent probe method,and between the BD960 liquid culture method and the direct smear fluorescence staining method(P =0.013,P＜0.001).Conclusion The PCR-fluorescent probe method and the BD960 liquid cul-ture method can quickly diagnose Mycobacterium tuberculosis(MTB)and nontuberculosis mycobacteria(NTM).How-ever,when MTB and NTM grow together,only MTB can be expressed,which has detection limitations and needs to be further improved.The detection rate of the direct smear fluorescence staining method is low,but the detection method is simple,rapid and cheap,and is suitable for preliminary identification in basic laboratories.
Keywords：MycobacteriumPolymerase chain reaction(PCR)-fluorescence probe methodBD960 liq-uid culture methodDirect smear fluorescence staining method
Publication Date：2023-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1282-1285 )