A study on the relationship between serum DNMT1 and lncRNA MEG3 expression levels and Parkinson's dis-ease

MA Ying; LI Qin-fu; QI Li-xia

doi:10.3969/j.issn.1674-3806.2023.12.13

Abstract：Objective To study the relationship between serum deoxyribonucleic acid(DNA)methyltrans-ferase 1(DNMT1)and long non-coding ribonucleic acid(RNA)human maternally expressed gene 3(lncRNA MEG3)expression levels and Parkinson's disease(PD).Methods One hundred and six patients with PD who were admitted to the Department of Neurology of Qinghai Red Cross Hospital from March 2020 to January 2023 were recruited as the PD group,and 103 cases undergoing healthy physical examination during the same period were selected as the control group.The clinical data were compared between the two groups to analyze the correlation between the serum DNMT1 and lncRNA MEG3 expression levels and clinical indicators in the PD patients.Multivariate logistic regression was used to analyze the factors affecting the occurrence of PD,and receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of the serum DNMT1 and lncRNA MEG3 levels in PD.Results The serum DNMT1 level in the PD group was higher than that in the control group,and the total cholesterol(TC),triglyceride(TG),low density lipoprotein-cholesterol(LDL-C)and lncRNA MEG3 levels in the PD group were lower than those in the control group,and the differ-ences were statistically significant(P＜0.05).The results of Pearson correlation analysis showed that the serum DNMT1 level of the PD patients was negatively correlated with TC,TG and LDL-C levels(P＜0.05),and was positively cor-related with the Unified Parkinson's Disease Rating Scale(UPDRS) Ⅰscores,UPDRS Ⅱ scores,UPDRS Ⅲ scores and Hoehn-Yahr(H-Y)staging(P＜0.05).The lncRNA MEG3 level was positively correlated with TC,TG and LDL-C levels(P＜0.05),and was negatively correlated with UPDRS Ⅰ scores,UPDRS Ⅱ scores,UPDRS Ⅲ scores and H-Y staging(P＜0.05).The results of multivariate logistic regression analysis showed that higher serum DNMT1 level was an independent risk factor for promoting PD occurrence(P＜0.05),and higher lncRNA MEG3 level was an inde-pendent protective factor for inhibiting PD occurrence(P＜0.05).The results of ROC curve analysis showed that the serum DNMT1 and lncRNA MEG3 levels had application value in the diagnosis of PD(P＜0.05),and the combina-tion of the two indicators could further improve the diagnostic efficiency[AUC(95%CI)=0.906(0.865-0.947)].The sensitivity and specificity were80.21%and89.34%,respectively.Conclusion Serum DNMT1 expressionis high in PD patients,and lncRNA MEG3 expression is low.The combined detection of the two indicators is helpful for clinical diagnosis of PD.