A study on the expressions of GPR15 and HMGA1 in colorectal cancer tissues and their clinical prognostic value

WANG Peng-hui; HU Jie; PAN Rong-yan

doi:10.3969/j.issn.1674-3806.2023.12.10

Abstract：Objective To investigate the expressions of orphan receptor G protein coupled receptor 15(GPR15)and high mobility group proteome A1(HMGA1)in colorectal cancer(CRC)tissues and their clinical prognostic value.Methods Eighty-nine CRC patients who underwent radical surgery in Affiliated Hospital of Yangzhou University from January 2019 to January 2020 were selected,and their intraoperative CRC tissues and the cancer-adjacent tissues were collected.Quantitative real-time polymerase chain reaction(qRT-PCR)and immunohistochemistry staining were used to detect the expressions of GPR15 and HMGA1 in the tissues.The correlation between the expressions of GPR15 and HMGA1 in the CRC tissues and the clinicopathological characteristics of the patients was analyzed.The Kaplan-Meier method was used to analyze the correlation between the expressions of GPR15 and HMGA1 in the CRC tissues and the patients'survival prognosis.Cox regression was used to analyze the influencing factors of prognosis in the CRC patients.Results The relative expression levels of GPR15 and HMGA1 mRNA in the CRC tissues were significantly higher than those in the cancer-adjacent tissues(P＜0.05).The positive expression rates of GPR15 and HMGA1 proteins in the CRC tissues were significantly higher than those in the cancer-adjacent tissues(P＜0.05).The positive expression rates of GPR15 and HMGA1 in the CRC tissues with TNM stage Ⅲ,low differentiation,and combined lymph node metas-tasis were higher than those in the CRC tissues with TNM stage Ⅰ-Ⅱ,high differentiation and non-lymph node metas-tasis,respectively(P＜0.05).The survival prognosis of the GPR15 negative group was significantly better than that of the GPR15 positive group(log-rank test:χ2 =9.124,P =0.003).The survival prognosis of the HMGA1 negative group was significantly better than that of the HMGA1 positive group(log-rank test:χ2 =10.140,P =0.001).Tumor TNM stage Ⅲ,low differentiation,combined lymph node metastasis,GPR15 positive and HMGA1 positive were the inde-pendent risk factors promoting poor prognosis of the CRC patients(P＜0.05).Conclusion The expression levels of GPR15 and HMGA1 are increased in CRC tissues,which are closely related to adverse clinicopathological character-istics of CRC patients.GPR15 and HMGA1 can be used as new tumor markers to evaluate the prognosis of the CRC patients.