Analysis on the correlation of serum lncRNA ROR and miR-145 levels with the occurrence of atrial fibrilla-tion in chronic heart failure patients

WU Hai-tao; LI Hong-jing; MIAO Yan-li

doi:10.3969/j.issn.1674-3806.2023.12.08

Abstract：Objective To analyze the correlation of serum long non-coding RNA(lncRNA) ROR and micro ribonucleic acid-145(miR-145) levels with the occurrence of atrial fibrillation(AF) in chronic heart failure(CHF) patients. Methods One hundred and eight CHF patients admitted to Handan First Hospital from March 2019 to December 2022 were recruited. The patients were divided into AF group(n=46) and non-AF group(n=62) according to the occur-rence of AF. During the same period, 50 healthy individuals who matched gender and age with the CHF patients were included as the control group. Quantitative real-time polymerase chain reaction(qRT-PCR) was used to detect the expres-sion levels of serum lncRNA ROR and miR-145. Pearson correlation analysis was used to explore the correlation of serum lncRNA ROR and miR-145 expression levels with cardiac function indicators. Multivariate logistic regression was used to analyze the influencing factors of AF occurrence in the CHF patients. The predictive efficacy of serum lncRNA ROR and miR-145 in the occurrence of AF was analyzed for the CHF patients using receiver operating characteristic(ROC) curve. Results Compared with those in the control group, the levels of lncRNA ROR in the non-AF group and the AF group were up-regulated, and the AF group had higher levels of lncRNA ROR than the non-AF group, with a statisti-cally significant difference(P ＜0. 05). Compared with those in the health group, the levels of miR-145 in the non-AF group and the AF group were down-regulated, and the AF group had lower levels of miR-145 than the non-AF group, with a statistically significant difference(P＜0.05). The results of Pearson correlation analysis showed that serum lncRNA ROR levels were positively correlated with left atrial diameter(LAD), left ventricular end diastolic diameter(LVEDD), and left ventricular end systolic diameter(LVESD)(P ＜0. 05), and negatively correlated with left ventricular ejection frac-tion(LVEF)(P ＜0. 05). The level of miR-145 was negatively correlated with LAD, LVEDD and LVESD(P ＜0. 05), and positively correlated with LVEF(P ＜0. 05). The results of multivariate logistic regression analysis showed that New York Heart Association(NYHA) classification cardiac function classⅣ, decreased LVEF, and up-regulated lncRNA ROR level were independent risk factors for promoting the occurrence of AF in the CHF patients(P＜0. 05), while up-regulated miR-145 level was an independent protective factor for inhibiting the occurrence of AF in the CHF patients(P ＜0. 05). The results of ROC curve analysis showed that the expression levels of serum lncRNA ROR and miR-145 could effec-tively predict the occurrence of AF in the CHF patients, and the combination of the two indicators had better predic-tive efficacy[AUC(95％CI) =0. 814(0. 730-0. 897), P ＜0.001], and the sensitivity and the specificity were 89.80％and 72. 00％, respectively. Conclusion Serum lncRNA ROR and miR-145 are closely related to the occurrence of AF in CHF patients. The combined detection of the two indicators has higher predictive value for the occurrence of AF.