Dynamic effects of rice wine on osteoblasts, osteoclasts and intraosseous pressure of rabbit femoral head

doi:10.3969/j.issn.1674-3806.2019.02.09

Abstract：Objective To observe the effects of rice wine on osteoblasts, osteoclasts and intraosseous pressure of rabbit femoral head. Methods Sixty-four rabbits were randomly divided into experimental group and control group according to random number table method, with 32 cases in each group. The experimental group was given rice wine in a dose of 10 ml/ ( kg·d) by inserting stomach tube, 3 times a week. The control group was given the same amount of normal saline in the same way. Eight rabbits were randomly selected from the two groups respectively before and 1, 3, 6 months after experiment. The rabbits were sacrificed by air embolization after the internal pressure of the femoral head of left hind limbs was measured, and then the femoral head of right hind limbs was taken as specimens.The specimens underwent fixation, decalcification, dehydration, embedding and other steps, cutting to 5 μm, HE staining, and the number of osteoblasts and osteoclasts were calculated under light microscope. Results There were no significant differences in the number of osteoblasts, osteoclasts and intraosseous pressure between the two groups before and one month after intragastric administration ( P＞ 0. 05). The number of osteoblasts was significantly less, and the number of osteoclasts was significantly more, and the intraosseous pressure was significantly higher in the experimental group than those in the control group 3 and 6 months after intragastric administration ( P ＜ 0. 05). Conclusion Long-term oral administration of rice wine ( more than 3 months) can decrease the number of osteoblasts, increase the number of osteoclasts and raise the intraosseous pressure in the femoral head of rabbits.