Application value of assessment for oosperm quality in predicting embryonic development potential and clinical pregnancy rate

doi:10.3969/j.issn.1674-3806.2019.01.12

Abstract：Objective To explore the application value of the pronuclear morphology score and the presence of cytoplasmic halo in predicting the embryonic development potential and clinical pregnancy rate.Methods In this retrospective study, 618 cases zygotes were assessed according to pronuclear scoring systems and checked for the formation of cytoplasmic halo.The embryo cleavage rate, day 3 frozen embryo rate, day 3 high-quality embryo rate, blastocyst formation rate, frozen blastocyst formation rate, high-quality blastocyst formation rate and the clinical pregnancy outcome were analyzed.Results The day 3 embryo cleavage rate, high-quality embryo rate, blastocyst formation rate, and frozen blastocyst formation rate were significantly higher in Z1, Z2, Z3 zygotes than in Z4 zygotes (P＜0.05).The blastocyst formation rate of Z1 was significantly higher than that of Z3 (P＜0.05).Compared with those in halo-negative zygotes, the embryonic development parameters were significantly higher than those in halo-positive zygotes (P＜0.05).The embryonic development parameters of halo-positive Z1, Z2, Z3 zygotes were significantly higher than those of halo-negative Z1, Z2, Z3 zygotes (P＜0.05), however, only the day 3 high-quality embryo rate was significantly higher in the halo-positive Z4 zygotes than in the halo-negative Z4 zygotes (P＜0.05).Whether Day3 embryos appeared or not, a halo at zygote stage was not significantly different in the clinical pregnancy rate and implantation rate (P＞0.05).Conclusion Pronuclear morphology score and the presence or absence of cytoplasmic halo can indicate the developmental potential of embryo in vitro, but the cytoplasmic halo can not predict the embryo implantation and clinical pregnancy rate effectively.