An experimental study of repairing the bone defects on rabbits by hyperbaric oxygen combined with purslane ;speedwell herb

doi:10.3969/j.issn.1674-3806.2016.04.02

Abstract：Objective To observe the curative effect of hyperbaric oxygen combined with purslane speedwell herb on treating bone defects, and to investigate the mechanism of the treatment.Methods 80 New Zealand rabbits were selected to make a bone defect model and then they were randomly divided into four groups.Group A was treated by hyperbaric oxygen combined with purslane speedwell herb.Group B received the therapy of hyperbaric oxygen. Group C was treated with purslane speedwell herb.Group D was taken as the control.The rabbits were sacrificed e-venly in each group at the end of the second, 4th, 8th and 12th weeks respectively.The general observation, X-ray, the detection under light microscope and electron microscope were performed, and the results were compared between the two treatment methods.Results X-ray showed that group A had the maximum amount of the formation of callus, followed by group B, C and group D.Under the electron microscope, osteoblast organelles in group A were more well-developed than those in group B, C and D, and the collagen fibers of group A arranged regularily and distributed evenly.Conclusion Hyperbaric oxygen combined with purslane speedwell herb is more effective in the treatment of bone defects than hyperbaric oxygen alone or herbal treatment alone.It can significantly enhance the ability of prolif-eration and the differentiation of osteoblast, and accelerate the growth of callus obviously, which provides a new meth-od for the clinical treatment of bone defects.