Effects of overexpression of miR-1284 on EIF4A1 in gastric cancer cells
Abstract：Objective To explore the potential target genes of miR-1284 and its possible molecular mecha-nisms on gastric cancer development.Methods The bioinformatics software was used to predict the target genes of miR-1284.The slow virus mediated miR-1284 transfection MGC-803 gastric cancer cells were taken as the miR-1284 group(LV-miR-1284).The empty carrier slow virus vector transfection cells were taken as the negative control group and the untransfected slow virus carrier cells as the blank control group.Fluorescence quantitative PCR was used to detect the expression of target genes EIF4A1and Western blot was used to detect EIF4A1 protein expression and its target genes were verified by dual luciferase EIF4A1.Results Compared with those in the negative control group and the blank control group, the expressions of gene and protein of EIF4A1 were decreased in the miR-1284 group, Dual luciferase test demonstrated that miR -1284 could combine with target genes EIF4A1 3′UTR.Conclusion miR-1284 plays a role in biological functions by combining with its target gene EIF4A1.
Keywords：Gastric cancermiR-1284EIF4A1
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 97-101 )