A hypetr enis on rat model induced by chronic intermittent hypoxia

doi:10.3969/j.issn.1674-3806.2015.11.01

Abstract：Objective To establish a hypertension rat model induced by chronic intermittent hypoxia, and to provide a simple and duplicate animal model for the study of the pathogenesis of hypertension in OSAHS.Methods The intermittent hypoxia system was used to conduct the experiment , including the control subsystem, the gas supply subsystem and the exposed cabin.24 male Sprague-Dawley rats, aged 8 to 10 weeks, and weighted from 200 to 250 g were randomly divided into two groups:the intermittent hypoxia intervention group and the control group.The inter-mittent hypoxia intervention group(n=12) was exposed to 6%to 8%oxygen for 40 seconds and then to 21 %oxy-gen for 80 seconds in the exposed cabin for 8 hours per day with many intermittent hypoxia cycles.The control group ( n=12) was exposed to certain concentration oxygen chamber.The tail artery systolic pressure of the rats was taken at rest states for three consecutive times and the average was calculated.Results The intermittent hypoxia interven-tion group had a significantly higher systolic blood pressure than the control group at the 35th day of the experiment [(136.21 ±32.46)mmHg vs (121.37 ±35.37)mmHg, P<0.05].Conclusion Precisely aerating the oxygen on and off in an certain range of concentration can effectively make a simulated intermittent hypoxia process, which is an ideal animal model for studying the mechanisms of intermittent hypoxia-induced hypertension.