Diffeer nt cultuer media, and inoculation density cultivatee ctomesenchymla set m cells, umbi lical cord blood
Abstract：Objective To explore the appropriate culture conditions of umbilical cord blood mesenchymal stem cells.Methods The sterile cord blood from normal full-term newborns was randomly divided into three groups:the high sugar DMEM group , the low sugar DMEM group and the umbilical cord blood mesenchymal stem cells be -tween groups .Extractions of umbilical cord blood mononuclear cells were inoculated in umbilical cord blood mesen -chymal stem cells in the culture media by the density of 1 ×105 , 1 ×104 , and 1 ×106 cells/ml respectively.The three kinds of media and different inoculation density culture adherent cell growth situations were observed and analy -sis of cell surface antigen was performed using flow cytometry technique .Results Ectomesenchymal stem cells of sugar DMEM group did not grow on the sidewall , while the growth of the spindle formation fiber sample cells was found on the sidewall in low sugar DMEM group and umbilical cord blood mesenchymal stem cell ( P<0.05 ) .Of dif-ferent inoculation densities , cells grew best at the density of 1 ×105 , followed by 1 ×106 .Cells failed to grow at the density of 1 ×104 .Conclusion Umbilical cord blood mononuclear cells in T 25 culture bottle grow best at the inocu-lation density of 1 ×105/ml, which is the most suitable culture medium for umbilical cord blood mesenchymal stem cell.
Keywords：Umbilical cord bloodMesenchymal stem cellIsolated culture
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 806-809 )