Reduction of nosocomial infection of MRSA in pediatric intensive care unit by real-time fluorescence PCR
Abstract：Objective To reduce nosocomial infection of meticillin-resistant staphylococcus aureus( MRSA) in pediatric intensive care unit(PICU) by real-time fluorescence PCR.Methods From July 2011 to June 2012, 856 specimens in PICU were detected for MRSA by using real-time fluorescence PCR and isolation-culture-K-B paper disk diffusion method.The nosocomial infection rate during the period from July 2009 to June 2010 was compared with that from July 2011 to June 2012.Results Thirty-three strains of staphylococcus aureus were identified by culture and re-al-time fluorescence PCR.Of the 33 clinical isolated staphylococcus aureus, 12 isolates were defined as MRSA by K-B paper disk diffusion method and 14 isolates were defined as MRSA by real-time fluorescence PCR.The two isolates which were defined as positive by real-time fluorescence PCR and negative by K-B paper disk diffusion method were proved to be MRSA positive by sequencing method.Nosocomial infection rates reduced from 36.4%, 37.5% to 16.7%.Conclusion The real-time fluorescence PCR can identify MRSA more rapidly and accurately and help re-duce the rate of nosocomial infection of MRSA in PICU.
Keywords：Methicillin resistant staphylococcus aureusReal-time fluorescence PCRNosocomial infec-tionPediatric intensive care unit(PICU)
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 538-540 )