Preliminary observation of homozygous deletions of RB1CC1 gene in disseminated cells of bone marrow of ;mouse oral carcinoma model
Abstract：Objective To observe the homozygous deletions of RB1CC1 gene of disseminated cells in bone marrow of mouse oral carcinoma model in the severe abnormal hyperplasia stage.Methods The cytokeratin positive ( CK+) cells from 35 single nuclear cells smear of bone marrow of mouse oral carcinoma model in the severe abnor-mal hyperplasia stage were isolated by Laser Capture Microdissection ( LCM) .The DNA of a single cell was ampli-fied by the technique of single-cell Whole Genome Amplification (WGA) and the homozygous deletions of RB1CC1 gene of the DTC were detected by Polymerase Chain Reaction ( PCR) .Four normal, 4 severe abnormal hyperplasia and 4 squamous cell carcinoma tongue were taken as control.Results Thirty five CK+cells from the 35 smear re-spectively were successfully isolated by LCM.Genomic DNA were successfully amplified by single-cell WGA in 3 out of 35 cells.Homozygous deletions of RB1CC1 gene were found in 2 of the 3 CK+cells and 2 in 4 cases of squamous cell carcinoma tongue mucosa.However, the deletion was not found in the rest tissues.Conclusion Homozygous deletion of RB1CC1 gene of the CK+positive cells in the severe abnormal hyperplasia stage may be the disseminated tumor cells.
Keywords：MouseDisseminated tumor cellsLCMRB1CC1 geneHomozygous deletion
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 394-397 )