Relationship between IL-1β-511 and IL-10-592 gene polymorphism and the susceptibility of pneumoconiosis in coal miners in Guangxi

doi:10.3969/j.issn.1674-3806.2015.04.01

Abstract：Objective To analyze the relationship between gene interleukin (IL)-1β-511, interleukin gene (IL)-10-592 polymorphism and genetic susceptibility of pneumoconiosis in coal miners in Guangxi .Methods A questionnaire surrvey of occupational history , history of pneumoconiosis etc , was conducted among 88 pneumoconiosis patients and 44 dust-exposed workers .The peripheral venous blood was collected in each studied object , and poly-merase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) technique was used to detect polymor-phism of interleukin gene IL-1β-511 and IL-10-592 .Results Eighty-eight pneumoconiosis patients and 44 dust-es-posed workers were the workers in a Bureau of Mines in Guangxi .Among 88 pneumoconiosis patients , there were 64 patients with silicosis who were exposed primarily to silica dust;24 patients with coal workers′pneumoconiosis who were exposed primarily to coal dust .Electrophoresis showed that wild-type gene of IL-1β-511 in silicosis group was 13 cases, 50 cases of mutant and wild type gene of IL-10-592 was 35 cases, 29 cases of mutant;wild-type gene of IL-1β-511 in Coal workers′pneumoconiosis group was 4 cases, 20 cases of mutant and wild type gene of IL-10-592 was 15 cases, 9 cases of mutant;wild-type gene of IL-1β-511 in the control group was 17 cases, 27 cases of mutant;wild type gene and mutant of IL-10-592 both were 22 cases.In comparison of IL-1β-511, IL-10-592 allele frequency in Coal workers′pneumoconiosis group and silicosis group comparison with that in the control group , the difference was not statistically significant ( P>0.05 ) .Conclusion The interleukin gene IL-1β-511 and IL-10-592 gene polymor-phism may be irrelevant to the incidence of Guangxi mine workers′pneumoconiosis .