Effect of p53 gene therapy on the local immunity and the prognosis in patients with nasopharyngeal carcino-ma

doi:10.3969/j.issn.1674-3806.2015.02.04

Abstract：Objective To investigate the local immune function and prognosis in patients with nasopharynge-al carcinoma( NPC) treated with p53 gene therapy.Methods Sixty-three cases of advanced nasopharyngeal carcino-ma were divided into p53 therapy group(n=32) and conventional therapy group(n=31).The p53 therapy group was treated by concurrent radiotherapy and chemotherapy plus nasopharynx intratumaral injection of rAd-p53;the con-ventional therapy group was treated by concurrent radiotherapy and chemotherapy.The expression of CD3 and CD20 were examined by immunochemistry and their relationship with prognosis was also analyzed.Results The expression of CD3 was significantly increased after p53 gene treatment.There was no significant change in expression of CD20 after p53 gene treatment.Five-year disease-free survival rate in the p53 therapy group was higher than that in the con-ventional therapy group( P<0.05 ) .Recurrence and metastasis rate was significantly different between p53 therapy group and the conventional therapy group(P<0.05).Conclusion T cells are the most important proliferative cell of tumor infiltrating lymphocyte in NPC patients after p53 gene therapy.The local cellular immune status in NPC pa-tients is improved after p53 gene therapy with decrease of tumor recurrence rate and metastasis rate.Gene therapy combined with radiotherapy and chemotherapy may help to improve the prognosis of patients with NPC.