A preliminary application of PCR-DGGE technology to research the changes of oral microbial floras in chil-dr en with hand foot and mouth disease
Abstract：Objective To apply PCR-DGGE technology analysing changes of oral microbial floras in children with hand foot and mouth disease( HFMD) .Methods According to the random number table method,3 children with HFMD were randomly chosen and their saliva samples were collected;at the same time, saliva samples were collected from one normal child,and from one child with herpetic ginggivostomatitis(HGS).Samples′total DNA extraction of the microbial community were performed;taking it as template for amplification of 16 s rRNA V3 variable area, the structure of product by the DGGE fingerprint, and using UVIBAND/MAP software for comparing similarity index of the community fingerprint;the edge banding on swimming belt of DGGE fingerprint were excised from the gel for se-quencing, gene sequence analysis was performed, then the sample advantage bacterium group was obtained.Results The advantage bacterial colonies of HFMD group:Capnocytophaga, Selenomonas, Prevotella, TM7_genera_incertae_sedis, Streptococcus, Neisseria, Porphyromonas, Campylobacter, Campylobacter, Granulicatell-a, Leptotrichia, Veil-lonella, Bacteroides-like sp, Actinomyces, Fusobacterium.The advantage bacterial colonies of normal group: Veil-lonella, Capnocytophaga, Prevotella,Gemella , Porphyromonas, Ruminococcaceae, Lachnospiraceae, Selenomonas, Clostridium, Haemophilus.The advantage bacterial colonies of HGS group: Lactococcus,Prevotella.Conclusion The flora structure of HFMD group was more complex, and there was obvious difference in the flora structure between HFMD group and HGS group.
Keywords：Polymerase chain reation-denaturing gradient gel electrophoresis(PCR-DGGE)Handfoot and mouth disease( HFMD)Herpetic ginggivostomatitis( HGS)
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 97-101 )