Development of method of detecting HIV antibody with chemically synthesized polypeptide antigen
Abstract：Objective To prepare diagnostic kit for HIV-Ⅰ/Ⅱ antibodies with mixed polypeptide antigen including HIV-Ⅰgp41, gp120, p24 and HIV-Ⅱ gp36.Methods The appropriate concentration of coated antigen and HRP-Ag were selected by checkerboard titration, and the sensitivity and specificity of the kit were conducted.Re-sults The appropriate concentration of coated antigen was 5 μg/ml, and the appropriate concentration of HRP-Ag was 1∶160.After optimizing the detection system, the CUT-OFF value, sensitivity and specificity of the kit were 0.207, 97.2%and 98.6%, respectively.Conclusion It is feasible that detect HIV infection via polypeptide anti-gen coated in polystyrene plate with high sensitivity and specificity.
Keywords：Chemically synthesized polypeptideAntigenELISAHIV-Ⅰ/Ⅱantibodies
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 899-902 )