Vasoactive intestinal peptide receptor mRNA expression in gastric adenocarcinoma
Abstract：Objective To investigate the expression of vasoactive intestinal peptide receptor ( VIPR) mRNA in gastric adenocarcinoma and AGS cells , and understand the effect of VIPR on gastric adenocarcinoma and possible mechanism.Methods Gastric tissues of 24 patients with gastric adenocarcinoma and 24 healthy controls were col-lected, and the expression of VIPR mRNA was determined by real time PCR .The VIPR mRNA expression was also detected in gastric adenocarcinoma cell line ACG by the same way .Results The expression rate of VIPR1 mRNA was 45.00%(9/24)in gastric adenocarcinoma, which was significantly lower than controls 83.33%(20/24)(P<0.05 ) , but that of VIPR2 mRNA didn′t changed significantly ( 50.00% vs 66.67% ) ( P>0.05 ) .The levels of VIPR1 mRNA in gastric adenocarcinoma was lower than that in controls ( P<0.05 ) , while there was no different in the levels of VIPR2 mRNA(P>0.05).All of the AGS cells were positive both in VIPR1 and VIPR2 mRNA expres-sion.Conclusion The lower level of VIPR mRNA expression may be an integral part in the pathogenesis of gastric adenocarcinoma , and VIPR mRNA detection may be contribute to the diagnosis of gastric carcinoma .
Keywords：Gastric adenocarcinomaVasoactive intestinal peptide receptormRNA
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 579-583 )