Expression of serum lipopolysaccharide binding protein before and after mechanical ventilation in thoracic surgery
Abstract：Objective To study the difference between the expression of lipopolysaccharide binding protein ( LBP) in serum before and after mechanical ventilation , explore the specific marker in early diagnosis of ventilator-induced lung ( VALI) injury and find out the target of clinical treatment .Methods Twenty patients who received rad-ical resection of lung cancer were selected .Every two lung cancer patients with the same surgical sites and methods were divided into the TLV group and the OLV group .Venous blood was collected at the moment before mechanical ventilation(T0), at the end of operation(T1),at 24 h after operation(T2) and at 48 h after operation(T3).After the blood was separated by centrifugation , the supernatant was collected and stored them at -80℃.Western blot meth-od was used to measure the LBP expression levels .Results The difference in the two groups was statistically signifi-cant at each time point ( P<0.05 ) , but the difference between the groups at each time point was not statistically sig-nificant(P>0.05), there was an interaction between the groups and each time points .The increased amplitude of LBP was different in the two groups ,the OLV group had a bigger rise than the TLV group .Conclusion Mechanical ventilation affects the LBP expression level , and the LBP indeed plays an important role in the VALI .
Keywords：Ventilator-associated lung injury ( VALI )Lipopolysaccharide binding protein ( LBP )Western blot method
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 498-501 )