The effects of overexpression and siRNA interference of the human hepatocellular carcinoma cell line HepG2 on the expression of 11β-hydroxysteroid dehydrogenase1
SUI Jing-zhe
LI Guo-ping
LI Shan
Abstract：Objective To design a adenovirus vector based system and specific siRNA for exploring the effects of overexpression and siRNA interference of the human hepatocellular carcinoma cell line HepG 2 on the ex-pression of 11β-hydroxysteroid dehydrogenase 1.Methods A adenovirus overexpression vector and specific siRNA targeting the 11β-HSD1 gene was designed and transfected into HepG 2 cells.Expression of 11β-HSD1 mRNA and protein was analyzed by real time PCR and Western blotting .Results A adenovirus transfection resulted in upgrade of 11β-HSD1 protein, and siRNA transfection led to inhibition of 11β-HSD1 mRNA and protein.Conclusion Trans-fection of the hepatocellular carcinoma cell line HepG 2 using a adenovirus vector and siRNA interference can signifi-cantly influence expression of 11β-HSD1 mRNA and protein .
Keywords：HepG2AdenovirussiRNA interference11β-HSD1
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 492-495 )