Studies on identificat ion of Blumea riparia (BI.) DC.by HPLC fingerprint
LEELAKITTISIN Benjakanr
JIANG Wei-zhe
HUANG Xing-zhen
Abstract：Obje tcive To establish qualitative chemical fingerprint of Blumea riparia ( BI.)DC.by high-performance liquid chromatography (HPLC).Methods Phenomenex?Synergi 4 u Polar-RP 80A(250 mm ×4.6 mm, 4 μm) column was used with 25℃column temperature then gradient elution was performed by the mobile phase con -sisted of methanol-solution of acetic acid in water(pH 2.8) at 256 nm detecting wavelength with 1 ml/min flow rate. Determination of 12 batches of fingerprint chromatogram of Blumea riparia (BI.) DC.were performed, followed by u-sing the computer-aided similarity evaluation system for similarity analysis of chromatograms .Results Medicinal ma-terials composed of 12 different batches were examined by specified method then we figured out its HPLC chemical fingerprint at 256 nm detection wavelength.Peaks in the chemical fingerprint of Blumea riparia ( BI.)DC.were eval-uatied and overall similarity index were analyzed and compared then 11 common peak were confirmed .This chemical fingerprint consist of 11 characteristic peaks including protocatechuic acid ( the second peak )and chlorogenic acid peak(the fourth peak).According to similarity index able to reflect its medicinal properties , our 12 batches′s simi-larity index more than 0.99 so these 12 batches are high quality medicinal materials .Conclusion The novel reliable quantitative chemical fingerprint analysis could be both foundation of quality assurance at industry level and be an im -portant step for further application of Blumea riparia ( BI.) DC.
Keywords：Blumea riparia(BI.) DC.Chemical fingerprintHigh-performance liquid chromatography ( HPLC)Identification
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 407-410 )