The adhesive effects of colorectal cancer cells SW480 by CLD1 gene transfection
Abstract：Objective To investigate the adhesive effects of colorectal cancer cells SW 480 by CLD1 gene transfection.Methods Cell homotypic adhesive tests and cell-matrix adhesive tests were performed on SW480CLD1 (SW480 by CLD1 gene transfection),SW480control(SW480 by pEGFP-C1 transfection) and SW480 cells.Results The result of homotypic adhesive tests showed that the number of homotypic adhesive SW 480 CLD1 cells were less than those of SW480 control ( P =0.000 ) and SW480 cells ( P =0.000 ) .No significant difference was detected between SW480control and SW480 cells(P=0.376).The sequence of number of adhesive cell were SW480CLD1 <SW480control and SW480.The result of cell-matrix adhesive tests showed that the number of SW 480CLD1 adhesive cells were more than those of SW480control(P=0.000) and SW480 cells(P=0.000), significant difference was detected.No signifi-cant difference was detected between SW480control and SW480 cells(P=0.334).The sequence of number of adhesive cell were SW480CLD1 >SW480control and SW480.Conclusion The homotypic adhesion was decrease between SW 480 cells by CLD1 gene transfection.The adhesion was increase between SW480 cells and collagen Ⅳmediated by CLD1 gene transfection .
Keywords：Colorectal carcinomaCLD1( claudin-1)Gene transfectionAdhesive tests
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 394-397 )