Effects of leflunomide on the proliferation and apoptosis of the lipopolysaccharide-induced HaCaT cells
Abstract：Objective To observe the proliferation and apoptosis of the lipopolysaccharide (LPS)-induced HaCaT cells in the presence of the active leflunomide metabolite A 771726 .Methods MTT was used to detect the level of cell proliferation;flow cytometry ( FCM) was used to detect apoptosis of HaCaT cells;inverted phase contrast microscope (IPCM) was used to observe morphologic changes .Results The A771726 significantly inhibited the pro-liferation of LPS-induced HaCaT cells , the inhibition effect was concentration-and-time dependent;the A771726 pro-moted cell apoptosis and the apoptosis rate increased with its concentration , the LPS group showed dense monolayer cells under an inverted microscope , as the concentration of the A 771726 increased , the volume of the cells got smal-ler and intercellular space got larger , and the more vacuoles was observed in the cells .Conclusion Leflunomide can inhibit the proliferation of the LPS induced HaCaT cells and expedite their apoptosis , which may be one of the mecha-nisms of leflunomide in the treatment of psoriasis .
Keywords：LeflunomideHaCaTPsoriasisApoptosis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 302-305 )