Preparation of acetyl resveratrol and its content determination by HPLC
Abstract：Objective To prepare of acetyl resveratrol and establish a high performance liquid chromatogra-phy(HPLC) method for its content determination .Methods Resveratrol and acetic anhydride as raw materials ,the reaction temperature was 120 ℃.The inertsil ODS-SP column(150 mm ×4.5 mm,5 μm) was used and the mobile phase was CH3CN-H2O(45∶55,V/V),the flow rate was 1.0 ml/min.The detection wavelength was 300 nm.The col-umn temperature was 25℃and the injective volume was 20μl.Results The reaction get a high purity of acetyl res-veratrol ,the yield was 90%.A good linear relationship was observed in the concentration range of 20.0~400.0μg/ml, with the correlation coefficient of 0.999 9 .The average recovery rate was 99.82% and RSD was 0.89%( n=6 ) . Conclusion The synthetic method is proved to be simple and the determination method was accurate and sensitive with high precision ,which can be applicable for the preparation of acetyl resveratrol and its content assay .
Keywords：High performance liquid chromatography ( HPLC)Acetyl ReseratiolSyntheticContent determination
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 285-287 )