Expression of CCR9 on peripheral blood CD4 +T lymphocytes in non-small cell lung cancer patients and its ;chemotactic activity

doi:10.3969/j.issn.1674-3806.2014.04.02

Abstract：Objective To investigate the expression of CC chemokine receptor 9 ( CCR9 ) on peripheral blood CD4 +T lymphocyte cells of non small cell lung cancer ( NSCLC), and the effect of CCR9 and its ligand (CCL25) on peripheral blood CD4+T lymphocytes.Methods The study was performed in 42 NSCLC patients and 30 healthy controls .Flow cytometry was employed to detect the T lymphocyte subsets and the expression of CCR 9 on peripheral blood CD4 +T cells.Peripheral blood CD4 +T lymphocytes were isolated from all cases with magnetic-acti-vated cell sorting method .The cell trans-membrane test with 24-transwell was used to detect the effect of CCR 9/CCL25 on the lymphocyte migration .Results Compared with control group , the T lymphocyte subsets in pre-opera-tive patients were higher than that in post-operative patients(P<0.05).The frequency of CD4 +CCR9+T lympho-cyte in pre-operative group was both significantly decreased compared with post-operative group(3.33 ±1.11 vs 6.57 ± 1.92,P<0.05) and controls(3.33 ±1.11 vs 11.06 ±1.37,P<0.05).In the induce of CCL25, the chemotactic indexes(CI) of CD4 +T lymphocytes from NSCLC patients (3.14) was significantly lower than that from healthy per-sons(3.83).After anti-CCR9 mAb pretreatment, the lymphocytes CIs of NSCLC patients decreased significantly to 0.62 as compared with untreated controls .Conclusion The expression of CCR9 is down-regulated on NSCLC periph-eral blood CD4+T lymphocyte cells .CCR9/CCL25 interactions mediate the migration of CD 4 +T lymphocytes in bodies .The decreased expression of CCR 9 in the CD4+T lymphocytes of patients with NSCLC could effect the ability of lymphocytes migration , which might involve in the mechanisms of tumor escape immune surveillance .Operation could reverse the change of CCR 9 expression on CD4 +T lymphocyte cells .CCR9 may be suggested to indicate immu-nologic reconstitution after therapy and operation .