Effects of duration of hyperglycemia on cellular immunity function in diabetic rats

doi:10.3969/j.issn.1674-3806.2014.03.08

Abstract：Objective To investigate the influence of the duration of hyperglycemia on cellular immune fun-tion in diabetic rat .Methods Animal model of diabetes mellitus was establish via continuously intraperitoneal injec -tion of streptozotocin .Twenty rats with successful modeling were randomly divided into 4 groups,each group include five rats.Group A:on the 4th day of successful modeling ,insulin was injected to control glucose between 8~12 mmol/L. Group B:After 8 d of successful modeling ,insulin was injected to control glucose .Group C: After 2 weeks of suc-cessful modeling ,insulin was injected to control glucose .Group D:After successful modeling , blood glucose did not controlled in entire experiment course .5 normal rats were used as control group at the same time .Peripheral blood cells were collected at 5 d, 8 d, 15 d and 4 weeks.The ratio of the CD4 +,CD8 +,CD4+/CD8 +,CD4 +CD25 +/CD4 +T cells were detected by FCM .Results ( 1 ) It showed no significant change of the ratio of CD 4 +, CD8 +, CD4 +/CD8+,CD4 +CD25 +/CD4 +T cells on 4 d, 8 d, 15 d and 4 weeks in group A and group B when compared with the control group.(2)In group C,there was a significant decrease of CD4 +T lymphocytes on 15 d,and the pro-portion of CD8 +T cell increased instead .The ratio of CD4 +/CD8+had a significant decrease ( P<0.05 ) .The ratio of the CD4 +T cells had significant increased between 15 d and 4 weeks after controlling glucose , but it was still obvi-ously lower than that of control group(P<0.05).(3)In group D,there was a significant decrease of CD4+T lym-phocytes on 15 d,and the proportion of CD 8 +T cell increased .The ratio of CD4 +/CD8 +also had a significant de-crease(P<0.05).(4)The ratio of CD4 +CD25 +/CD4 +T cells hadn′t a significant difference between experimental groups and control group ( P>0.05 ) .Conclusion Diabetic rats have cellular immune dysfunction mediated by hy-perglycemia with duration time more than two weeks .It means that chronic hyperglycemia have great effect on cellular immune function .