Construction of eukaryotic expression ev tc ors of CCL11 gene 3′UTR and CCL26 gene 3′UTR and it′s signifi-cance
WENZ hih-ong
DAI Yan
HE Shuang
Abstract：Objective To construct the eukaryotic expression vectors of 3′untranslated regions(3′UTR) of CCL11 gene and CCL26 gene.Methods Target fragments in 3′UTR of CCL11 gene and CCL26 gene were amplified from genomic DNA by Polymerase chain reaction ( PCR) .The target fragments and pMIR REPORT vectors were doub-le cut by restriction enzyme ,and then the fragments were inserted into the pMIR REPORT plasmid to construct recom-binant plasmid .The recombinant plasmid of pMIR REPORT-CCL11 3′UTR and pMIR REPORT-CCL26 3′UTR were transformed DH5αcompetent cells,respectively.Results The 3′UTR sequence of CCL11 gene and CCL26 gene were successfully cloned into the pMIR REPORT vector ,which verified by restrictive enzyme digestion and DNA sequen-cing.Conclusion The recombinant plasmid of pMIR REPOR-CCL11 3′UTR and pMIR REPORT-CCL26 3′UTR were successfully constructed ,which lay a foundation of the further study of the relation between CCL 11 gene,CCL26 gene and microRNAs .
Keywords：CCL11 geneCCL26 gene3′UTREukaryotic expression vectorpMIR REPOR
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )