Comparative analysis of white blood cells in urine determined by automatic instruments and artificial micros-copy

doi:10.3969/j.issn.1674-3806.2013.11.10

Abstract：Objective To compare the result of white blood cells in the urine by the automated urine analy-zer( dry chemical method ) , automatic urinary sediment analyzer and artificial microscopy .Methods Three different methods ( dry chemistry method , urinary sediment method and artificial microscopy ) were used for determination of white cells in 845 urine samples ,and the results were compared .Results Using the microscope detection results as the gold standard in 845 urine samples , the detection of dry chemical method for urine leukocyte showed the positive coincidence rate was 89.69%,the negative coincidence rate was 66.82%, the false positive rate was 33.12%, the false negative rate was 10.31%, the positive likelihood ratio was 2.71 , the negative likelihood ratio was 0.15 , the total coincidence rate was 72.07%, comparing the results of the two methods , the difference was statistically signifi-cant( P<0.01 ) .The detection of urinary sediment method for urine leukocyte showed the positive coincidence rate was 95.87%, the negative coincidence rate 83.56%, the false positive rate is 16.44%, the false negative rate was 4.13%, the positive likelihood ratio was 5.80 , the negative likelihood ratio was 0.05 , the total coincidence rate 86.39%, comparing the results of the two methods , the difference was statistically significant ( P<0.01 ) .The com-parison between the dry chemical method and urinary sediment method showed the positive coincidence rate was 89.41%, the negative coincidence rate 76.81%, the false positive rate is 23.19%, the false negative rate was 10.59%, the total coincidence rate was 81.18%, the positive likelihood ratio was 3.85 ,the negative likelihood ratio was 0.13, there was significant statistically differences between two methods (P<0.01).Conclusion Urine visible components are complex , full automatic instrument method can not completely replace the microscopy and can only be used as a sieving mothod in analysis of large quantities of samples , so its positive test results still need to use the mi-croscopy to improve accuracy in reporting results .The combination of the three kinds of methods can improve the ac-curacy of the detection results and have great value in clinical application .