Analysis of KEGG-based differential metabolite-related signaling pathways in papillary thyroid cancer
Man Guodong
Wang Jianli
Wang Jun
Wang Juan
Abstract：Objective To investigate KEGG-based differential metabolite-related signaling pathways in papillary thyroid cancer(PTC).Methods Thirty PTC patients who underwent surgery at the Head and Neck Surgery Department of Gansu Provincial Cancer Hospital from April 2019 to March 2022 were selected,and the surgical samples were grouped according to the focal tissues(Group C)and the paracancerous thyroid tissues(Group NC),and the metabolites were extracted separately and then separated by using ultra-high-performance liquid chromatography,and then analyzed by using mass spectrometry to analyze the metabolites in the tissues of the samples of the two groups.The acquired significantly different metabolites were imported into the KEGG database for correlation pathway analysis.Results Metabolites such as Pidotimod,L-Proline,Propyzamide,Tyrosine,and Deoxysappanone B 7,4'-Dimethyl Ether(Deox B 7,4)were up-regulated in PTC lesion tissues(all P<0.05),and metabolites such as L-Norvaline,Bisphenol S,Leucine,(S)-3-Amino-4-Phenylbutyric Acid,and Isoleucylisoleucine expression were downregulated(all P<0.05).Among the significant difference metabolites involved in the metabolic pathway,three transcription factors,c-MYC,HIF-1 and p53,were the key regulators of PTC metabolism.These significantly different metabolites were mainly involved in protein digestion and absorption,mammalian target of rapamycin(mTOR)signaling pathway,mineral uptake,carbon metabolism in cancer centers,amino acid biosynthesis(including phenylalanine and arginine synthesis),aminoacyl-tRNA biosynthesis,alanine,aspartate,and glutamate metabolism,ABC transport,and other metabolic pathways.Conclusion Papillary thyroid carcinoma is characterized by the presence of 153 significantly different metabolites,which may affect the occurrence and development of PTC by regulating amino acid metabolism and mTOR signaling pathways to modulate related protein synthesis and expression.This provides new perspectives and potential diagnostic and therapeutic options for PTC metabolic abnormalities.
Keywords：Papillary thyroid carcinomaKEGGMetabolitesSignaling pathway
Publication Date：2024-07-27
Online Publishing Date：2025-11-27(First online date of this platform, not the publication date of the document)
Pages：6( 47-50,30,后插2 )