Clinical application of 2D-LC/UV determination of phenytoin plasma concentration
YUAN Jiang-li
ZHANG Han-juan
YUAN Dong-dong
Abstract：Objective: To establish a method for the determination of phenytoin in serum by two-dimensional liquid chromatography (2D-LC/UV) and improve the monitoring level of therapeutic drugs. Methods: The chromatographic column of one-dimensional liquid chromatography, trap column and two-dimensional liquid chromatography were Aston SC2 (4.6 mm × 25 mm, 5 μm), Aston SH (3.0 mm × 10 mm, 5 μm) and Aston SCB (4.6 mm × 100 mm, 5 μm) respectively. The mobile phase of one-dimensional liquid chromatography, trap column and two-dimensional liquid chromatography were VCV-1D (flow rate was 0.7 mL·min-1), pure water and mixed mobile phase (BPI-1 basic mobile phase : API-1 acidic mobile phase : MPI-1 mobile phase = 11 : 37 : 52, flow rate was 1.2 mL·min-1) respectively. The column temperature was maintained at 40 ℃. The UV absorption wave length was set at 240 nm. Results were calculated by external standard method. Results: Phenytoin showed good linearity at the range of 1.54 – 49.3 μg·mL-1 (r = 0.999 9). Phenytoin had a complete chromatographic peak within 7 minutes. The recovery rate was higher than 94%. RSDs of intra-day and inter-day precision were all less than 3.53%. RSD of the stability test was less than 5%. Results of serum samples from 30 patients taking oral phenytoin sodium showed that the serum concentration of phenytoin of 36.7% of the patients were out of the treatment window. Conclusion: This method was fast and accurate. It has high automation, good stability, which was suitable for the serum concentration monitoring of phenytoin.
Keywords：Two-dimensional liquid chromatographyPhenytoinTherapeutic drug monitoring
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 277-280 )