Pharmacokinetic study of S-oxiracetam in cerebral ischemia-reperfusion model rats
LI Ling
HU Xiu
LI Li
Abstract：Objective: To establish the HPLC method for the determination of S-oxiracetam in rat plasma and to figure out the pharmacokinetic differences between the normal rats and the cerebral ischemia-reperfusion model rats. Methods: Aniracetam was used as internal standard. The concentrations of S-oxiracetam in rat plasma were measured by HPLC-UV, which was performed on a APS-2 HYPERSIL column (250 mm × 4.6 mm, 5 μm) with the mobile phase of water-acetonitrile (12 : 88) at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 214 nm and the column temperature was 25 ℃. The pharmacokinetic parameters were calculated by DAS 2.0 software. Results: The calibration curve of S-oxiracetam was linear in the range of 0.5 – 100.0 μg·mL-1 (r2= 0.996 8). The mean recoveries, precision, accuracy and the stability were in line with the biological sample measurement requirements. The AUC 0-t, AUC0-∞, MRT0-t, t1/2z, CLzand Vzof S-oxiracetam in normal rats were significantly different from those in cerebral ischemia-reperfusion model rats. Conclusion: The method was proved to be sensitive, accurate, simple and had strong specificity, which was suitable for the pharmacokinetic study of S-oxiracetam. Compared with normal group rats, the AUC of S-oxiracetam increased and the elimination rate of S-oxiracetam slowed down in cerebral ischemia-reperfusion model rats.
Keywords：S-oxiracetamHigh performance liquid chromatographyCerebral ischemia-reperfusionPharmacokinetics
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 270-274 )