Concentration determination of levetiracetam in human plasma by HPLC
CAI Le
ZHU Ying
ZHANG Ming-hua
CHEN Jing-jing
CHAI Dong
Abstract：Objective: To establish a high-performance liquid chromatographic (HPLC) method for the concentration determination of levetiracetam in human plasma. Methods: With metronidazole as the internal standard, acetonitrile was added to plasma sample for precipitating protein. The supernatant was washed with dichloromethane, and the upper aqueous was injected to HPLC. Hypersil BDS column (4.6 mm × 250 mm, 5 μm) was selected. And the mobile phase was a mixture of water and acetonitrile (87 : 13, v/v) at a flow rate of 1.0 mL·min-1. The detection wavelength was 205 nm. Column temperature was 40 ℃ and injection volume was 20 μL. Results: Levetiracetam showed a good linear relation in the range of 2.5 – 80.0 μg·mL-1with a good correlation coefficient (r = 0.999?4). The intra-batch variations were ranged from 1.06% to 1.97% and inter-batch variations were ranged from 3.46% to 8.14%. The mean recoveries were ranged from 94.00% to 102.48%. Conclusion: The method was sensitive, specific and simple. It is suitable for monitoring the concentration of levetiracetam in human plasma.
Keywords：LevetiracetamMetronidazoleHPLCPlasmaTherapeutic drug monitoring
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 145-147 )