The effect of ethanol extracts from Spatholobus suberectus and its active component catechin on JAK/STAT pathway in spleen of radiation injured mice
DONG Xian-zhe
WANG Qi-shan
NIU Ke
TAN Xiao
GUO Dai-hong
LIU Ping
Abstract：Objective: To study the effect of ethanol extracts from Spatholobus suberectus and its active component catechin on JAK/STAT pathway in spleen of radiation injured mice. Methods: ICR mice were randomly divided into normal group, model group, positive control group (amifostine, 43.6 mg·kg-1, iv, 30 min before irradiation), SSD group (10, 20, 40 g·kg-1) and catechin group (50, 100 mg·kg-1). Except for normal group, all mice were exposed to 6.0 Gy 60Coγ irradiation with a dose rate of 117.29 cGy·min-1and a duration of 290 s. The mice were administered the appropriate drugs once a day after irradiation for 28 consecutive days. Blood samples were collected from the tail end and the number of peripheral blood cells was counted with a microcell counter before irradiation and on the 28thday. On the 28thday after irradiation, total proteins in spleen cells of mice were detected to study the expression of related protein using Western-blotting and total RNA in spleen cells of mice to show the expression of related gene with RT-PCR method. Results: Spatholobus suberectus and catechin both show activating-hematopoiesis effect. Compared with normal group, related protein in JAK/STAT signal pathway (P < 0.05) and the expression of mRNA (P < 0.01) were significantly up-regulated, the expression of mRNA of Bax and Caspase-3 markedly reduced, and the expression of Bcl-2 distinctly promoted in both Spatholobus suberectus and its active component catechin groups. Conclusion:Spatholobus suberectus and its active component, which have a certain ability to repair the spleen cells of mice, could activate JAK/STAT signal channel and inhibit apoptosis of spleen cells in mice with radiation injury.
Keywords：IrradiationSpatholobus suberectusSpleenJAK/STAT pathwayApoptosis gene
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 72-76,84 )