Quality standard studies of Tongnaoye
WANG Xiu-qin
QIAN Qiu-yu
WANG Dong-xing
SHI Lei
CHEN Jun
Abstract：Objective: To establish the quality standard of Tongnaoye. Methods: Thin layer chromatography (TLC) was used to identify crocus sativus in the prescription. High performance liquid chromatography (HPLC) was used to determine the content of crocin Ⅰ and crocin Ⅱ. Chromatographic conditions were as follows: Agilent ZORBAX SB-C18 column (250 mm × 4.6 mm, 5 μm) was used, the mobile phase consisted of methanol-water (48 : 52) with the flow rate of 1.0 mL·min-1, the detection wavelength was set at 440 nm, the temperature was 30 ℃ and the sample volume was 10 μL. Results: The TLC method showed good specificity and separation effect. CrocinⅠshowed a good linear relationship (r = 1) in the range of 9.66 - 57.94 μg·mL-1, while crocinⅡ showed a good linear relationship (r = 0.9999) in the range of 5.91 - 35.48 μg·mL-1. Their average recovery rates were 98.4% (RSD = 1.21%, n =9) and 99.4% (RSD = 1.70%, n = 9) respectively. Conclusion: This method was simple, accurate and reproducible, which could be used to control the quality of Tongnaoye.
Keywords：SaffronCrocin ⅠCrocin ⅡHPLC
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 271-274 )